Supplementary material for this article is available at http://advances.sciencemag.org/cgi/content/full/3/10/e1701350/DC1

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Primers PlcytbF2/ PlcytbR2, which were designed to amplify the cytb gene in P. litchii, produced a single bright band of 935 bp with both gDNA and cDNA (wild-type isolate 38). This fragment included possible mutations that can confer resistance to QoI fungicides (amino acid residues 120–160 and 250–300)27. The same primers were also used for the QoI fungicide-resistant mutants and their parents that are listed in Table 1, and they all yielded the same amplification products of 935 bp. The sequencing of these products revealed several point mutations in the QoI fungicide-resistant mutants (Fig. 2) (Table 2). In mutants M11-4 and S38, the amino acid substitution of glycine with alanine was detected at position 142 (G142A) of the CYTB protein. In mutants B0908 and BU, the substitution of glycine with serine was detected at the same position (G142S). A second target-site mutation, the substitution of phenylalanine with serine (F128S), was detected at position 128 in mutants A11-4 and A0902. The substitution of tyrosine with cysteine (Y131C) was detected at position 131 in mutants SU1 and SU2.

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20 samples from top U.S. brands (Figure 2) were analyzed. Due to the possibility of extreme foaming, samples were run in the “soil mode” of the 4100, where they are purged in a 40 ml vial instead of in a frit sparger.

After 45 min of cell culture, cell lysates [radioimmunoprecipitation assay (RIPA) buffer] were used to perform immunoprecipitation of integrin β1 and the BMP-2 receptor BMPRIa. Proteins (15 μg) were measured using NanoDrop (Thermo Fisher Scientific). First, an integrin β1 antibody (Abcam, 183666; 10 μl per reaction) was used together with Protein A-Agarose beads (Santa Cruz Biotechnology, sc-2001). Second, after denaturation and elution of beads, the samples were run in NuPAGE 4-12% bis-tris gels (Life Technologies). A mouse BMPRIa antibody was used to probe the membrane (Abcam, 166707; 1:1000). An anti-mouse secondary antibody was used (Santa Cruz Biotechnology, sc-2031; 1:5000). The enhanced chemiluminescence detection system (GE Healthcare) was used before the blot was exposed to x-ray.

The Washington Post: Breast Feeding Offers Some Surprising Benefits For Babies And Mothers A mother’s milk, rich in an exquisitely tailored mix of fats, proteins, vitamins and minerals, provides all the nutrition a helpless infant requires — and more. Breast milk is thought to protect against disease, set up a healthy digestive system and even influence a child’s behavior. And yet we know a lot less about this important substance than we could, says lactation researcher Katie Hinde of Arizona State University. (Wessel, 10/6)

his is out there, but: Try making borosilicate on Mars. Sourcing a high-quality silica source and heating it to the temperatures required is a tall order. This provides an option in-situ for production of rare drugs that MC couldn’t foresee needing to ship, or which would face expiration problems far away from a steady supply, and might require less energy and water if the reactor materials can be efficiently purified and recycled. Making more pp from ethanol to replace stock contaminated beyond recovery is a stretch, granted, but if they’re shipping a pp-capable printer, it’s probably being put to other use and there’s enough to go around. Yes, you could just ship glassware (how much is enough?), but this could still be a pretty nice backup for inevitable breakage.

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The Baltimore Sun: How To Get Naloxone, The Antidote For An Opioid Overdose, In Maryland As the opioid epidemic continues to generate staggering numbers of fatalities, Marylanders might consider whether to stock up on an antidote to overdoses that’s called naloxone. The medication comes in the form of a nasal spray, called Narcan, or an injection, called Evzio. The FDA-approved prescription medications can counteract the life-threatening effects of an opioid-related overdose. (Reed, 10/5)

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