The big picture is that there are three factors that we think are all pulling up that dividend growth upwards however, PHP’s historic dividend cover is 97%, not quite 100%, so it’s likely to want to get it more highly covered up to that 100% before accelerating the payout.

In addition, the Method 624 has a relatively limited list of analytes. Method 624.1 is a performance-based method. Latest technologies such as more sensitive GC/MS instruments, optimized instrument parameters, better purge and traps, and capillary columns will allow laboratories to achieve better precision and % Relative Standard Deviation (%RSD) for calibrations when compared to the prescribed 35% in Method 624 and 624.1. Enabling such high RSD values on majority of compounds usually indicates that the analytical system is uncontrollable and the associated data is likely to be suspect. The analyte list for 624.1 has been expanded and includes many compounds that can also be run by 8260.

The critical importance of protein structure in determining cell behavior is known (40–42), but translating this strategy into clinically relevant therapies has been challenging. Here, we describe a facile yet robust engineering approach that allows reliable control of synergistic integrin/GF signaling to promote stem cell differentiation and tissue repair. Notably, PEA can be readily applied to diverse biomedical devices, both planar and complex 3D geometries by spin coating, solvent casting, and plasma polymerization (43). We demonstrate that FN nanonetworks, simply adsorbed onto PEA from a solution, allow for simultaneous availability of the integrin-binding region (FNIII9–10) and the GF-binding region (FNIII12–13) (44) of this important ECM protein. The FNIII12–14 region is classically described as the heparin-binding region shown to be a promiscuous GF-binding region able to sequester different GF families [for example, platelet-derived growth factor (PDGF), fibroblast growth factor (FGF), and transforming growth factor–β (TGF-β)] (13). As a proof of concept, we show here that BMP-2 is bound onto the FN nanonetworks at the material interface, and this binding/presentation promotes enhanced BMP-2 signaling in MSCs, with very low doses of BMP-2 driving osteogenesis and complete repair of nonhealing defects in vivo. We show, for the first time, a surface-induced regeneration of the bone (volume) in a critical-size defect, emphasizing clinical potential. Previous studies have shown the importance of FN conformation in driving osteogenic differentiation on material surfaces, with correct FN conformation required to allow enhanced availability of the integrin-binding site (41). Coating implants with FN fragments leads to better osteointegration, which reveals the importance of integrin specificity–related mechanisms also in vivo (45). Our results show a moderate effect for fibrillar FN nanonetwork presentation on PEA to induce osteogenesis both in vitro, as previously reported (37), and in vivo (Fig. 3). This may be related to the presence of GFs in the regenerative environment (medium/serum), which are adsorbed on FN nanonetworks to induce synergistic signaling.

AFM examination showed that BMP-2 was preferentially adsorbed on top of FN molecules on PEA, whereas on PMA the GF appeared adsorbed over the polymer surface (Fig. 1D). Bare PEA and PMA surfaces are shown in fig. S1, and BMP-2 molecules directly adsorbed on PEA and PMA (without FN) are shown as individual globules in fig. S2. Figure 1D includes molecular-scale AFM phase magnitude images of individual BMP-2 molecules sequestered onto open FN dimers (32). The FN open conformation sequestration of BMP-2 molecules was assessed using an anti–BMP-2 antibody and then a secondary antibody labeled with a gold nanoparticle (Fig. 1E). Individual FN molecules on PEA were observed using phase magnitude, whereas the gold nanoparticles were depicted using height magnitude. A high-resolution AFM image of one of these FN molecules in an extended conformation adsorbed on PEA with one gold nanoparticle per FN arm is shown in Fig. 1E; the section shows a height profile of ~7 nm that is compatible with the dimensions of the gold nanoparticles buried within the antibody cluster. These results demonstrate that FN adsorption onto PEA, but not PMA, results in controlled binding and presentation of BMP-2 to the adsorbed FN molecules.

Halgren, T. A. Merck molecular force field .1. Basis, form, scope, parameterization, and performance of MMFF94. J. Comput. Chem. 17, 490–519 (1996).

Acrylic acid & esters is the general term for acrylic acid (AA) and acrylic esters (AE), wherein, AE mainly refers to common acrylic esters (CAE), inclusive of methyl acrylate (MA), ethyl acrylate (EA), butyl acrylate (BA), ethyl hexyl acrylate (EHA), etc.

CRRC Corporation Limited (CRRC), headquartered in Beijing, the world’s largest supplier of rail transit equipment with the most complete product lines and leading technologies, introduced its new generation carbon fiber metro vehicle “CETROVO” at the Innotrans 2018 in Berlin.

Yeah. Hey, thanks. Hey, Wendell, I must say, solid results here. My question is not on Optical this time; it’s on auto glass. Continue to hear lot of news flow around AutoGrade Glass. You’re getting into Automotive Interiors. Talk to us about how that demand — how that business kind of unravels heading into the rest of the year. Thanks.

TUI AG (LON:TUI), Prior to entering its close period ahead of reporting its full year results for the twelve months ending 30 September 2018 on 13 December 2018, TUI Group announced the following update on current trading.

Cells (B16F0s and hMVECs) were fixed using 4% paraformaldehyde for 20 min and permeabilized with 0.1% Triton X-100 for 30 min at room temperature. Bovine serum albumin (BSA; 1%) was used to block cells for 15 min, and then cells were stained with the appropriate primary and secondary antibodies (table S2). Before every step, cells were rinsed at least twice with PBS. Imaging was done using an LSM 700 (Carl Zeiss Inc.) four-laser point-scanning confocal microscope with a single pinhole for confocal imaging or an IN Cell Analyzer 2000 (GE Healthcare) for fluorescence imaging. Red and green cell trackers (Invitrogen) were used for coculture experiments on precultured B16F0s and hMVECs, respectively, as per the manufacturer’s instructions. Cells with cell trackers were fixed as described previously.

The journal article can be found here: https://academic.oup.com/ibdjournal/advance-article-abstract/doi/10.1093/ibd/izy281/5095645

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